Выходные данные
Статус 4th year undergraduate student
Организация St.Petersburg State University
Подсекция Аналитическая химия
Метки студент
Extraction into deep eutectic solvents as an alternative to the microwave decomposition of plant samples for elemental analysis
Gerasimov Artur Ivanovich
Dear Artur, in the motivation part you declare that «this approach completely eliminates need to use … acids», and then in the Figure you show the stage of adding 5 mL HNO3 to your sample. Is it correct? What are the future steps planned in your research work?
Dear Andrey, you have correctly noticed that in this work we used 5 ml of 0.1 M nitric acid to dissolve DES. Unfortunately, we have not been able to completely eliminate the use of acids. However, compared to microwave destruction method, which requires the use of large volumes of concentrated nitric acid and hydrogen peroxide, we suggest using dilute acids in this technique.
Also, in the future, we plan to study the interfering effect of various components of the tobacco matrix on the analysis of metals in the sample and extend the developed method to other plants, for example, lettuce leaves. We hope we have given a complete answer to your question.
Could you explain how trueness and precision of measurement were carried out.
As well, what equipment did you use in work? What measurement conditions have you chosen?
Ivan, thank you for your questions!
The trueness and precision of the developed sample preparation scheme was assessed by the t- and F-criteria in comparison with the test technique, which was microwave decomposition.
The measurements were performed using an ICP-OES system (ICPE-9000 instrument, Shimadzu, Japan) equipped with highly sensitive CCD detector. The samples were introduced with a concentric nebulizer and a cyclonic spray chamber. In all the measurements, argon gas (99.996%) was used to generate the plasma. Microwave-assisted acid digestion was performed in a microwave oven MDS-10 Sineo (China).
The optimal conditions for the developed technique are presented below:
DES composition — Choline chloride/Malic acid (1/1; mol./mol.)
Mass of DES/Mass of sample — 5/1
Extraction time — 30 min
Extraction temperature — 70°C
We hope we have given a complete answer to your question.
Not quite.
1. ISO 5725-1:1994(en) Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
2. I’d like to see parameters:
RF power (kW), Plasma flow (L/min), Axial flow (L/min), Nebulizer flow, Replicate read time (s), Plasma stabilization delay (s), Replicates; As well, sample introduction settings: Sample uptake delay (s), Pump rate (rpm), and emission lines used for ICP–AES analysis.